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Training in Advanced Testing Techniques for Dengue Virus

Enhance your laboratory skills with practical training in RT-PCR and Elisa techniques for dengue virus diagnostics. Discover good laboratory practices and biosafety measures that ensure accuracy and safety in testing.

August 21, 2025
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Training in Advanced Testing Techniques for Dengue Virus

Comprehensive Training in Dengue Virus Testing Techniques

Introduction to the Training

The recent training led by Dr. Atul Garg at SGPGI's Viral Research and Diagnostic Laboratory (VRDL) focused on essential laboratory skills for accurate dengue virus diagnostics. This program was designed to equip participants with theoretical knowledge and hands-on experience in various testing techniques.

Key Learning Outcomes

Participants engaged in a thorough learning experience that included:

  • Practical Demonstration of Elisa Technique:

  • An in-depth introduction to the Enzyme-Linked Immunosorbent Assay (Elisa), which is crucial for detecting antibodies in patients suspected of having dengue.

  • Molecular Testing Techniques:

  • RT-PCR Training: Focused on Reverse Transcription Polymerase Chain Reaction (RT-PCR), a vital tool for dengue virus serotyping, enabling rapid and reliable identification of active infections.

  • Good Laboratory Practices:

  • Emphasis was placed on adhering to standardized protocols that ensure the accuracy and reliability of test results.

  • Biosafety Practices:

  • Comprehensive training on biosafety measures was provided to ensure that all laboratory personnel operate in a safe environment, minimizing contamination and exposure risks.

  • Quality Control Measures:

  • Participants learned about implementing quality control processes that safeguard the integrity of laboratory results and maintain high testing standards.

Conclusion

This training not only enhanced the participants' diagnostic skills but also fostered a deeper understanding of laboratory safety and quality assurance practices. With advancements in techniques like RT-PCR and Elisa, health professionals are better equipped to tackle dengue virus outbreaks effectively and ensure patient safety. This initiative by SGPGI's VRDL underlines the importance of continuous education and training in the ever-evolving field of medical diagnostics.

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